Tissue Stimulators

Tissue stimulators are advanced biorevitalizing injectables used in aesthetic cosmetology and aesthetic medicine to restore skin structure and improve density, elasticity, and firmness. Their action is based on fibroblast activation and physiological stimulation of collagen, elastin, and extracellular matrix synthesis—without producing a traditional volumizing or filling effect.

This lesson provides an in-depth overview of the main groups of tissue stimulators, including hyaluronic acid–based products, hybrid HA complexes (e.g., Profhilo), amino acid formulations (e.g., Sunekos), and polynucleotides (e.g., Nucleofill, Vitaran). The course explains their mechanisms of action, biological differences between products, injection techniques (including the BAP method), as well as principles of client qualification and procedural safety.

The training includes theoretical content, instructional videos, step-by-step protocols, and concludes with a certificate of completion.

Learning Outcomes

After completing the lesson, the participant is able to:

• differentiate types of tissue stimulators: hyaluronic acid–based, hybrid HA, amino acid–based, collagen-supporting, and
• polynucleotide formulations
• explain the definition of tissue stimulators from a biorevitalization perspective
• classify biostimulators according to their mechanisms of action
• understand the cellular-level biological basis of tissue stimulator activity
• explain the role of fibroblasts in skin biorevitalization and remodeling processes
• interpret regenerative processes occurring in the skin following injection
• characterize the effects of polynucleotides in terms of regeneration, hydration, and skin quality improvement
• discuss the role of amino acids in stimulating collagen and elastin synthesis
• explain the mechanism of skin bioremodeling using hybrid hyaluronic acid
• analyze treatment areas in skin-quality–focused therapies
• apply knowledge of general facial anatomy
• consider skin anatomy and histology when planning injections
• identify skin conditions and blood-borne diseases that constitute contraindications
• perform skin assessment and properly qualify the client for treatment
• select the appropriate product based on age, skin type, and aesthetic concern
• plan an individualized treatment protocol, including combination therapies
• determine treatment frequency and number of sessions according to the biostimulator used and desired outcomes
• prepare the workstation, products, and equipment in accordance with aseptic standards
• select needles or cannulas appropriate to the injection technique
• perform tissue stimulator injections using the BAP (Bio Aesthetic Points) technique
• understand the rationale of BAP points and their anatomical placement
• monitor treatment outcomes and avoid volumetric overcorrection
• recognize typical post-procedure reactions and assess the course of skin recovery
• apply safety precautions and manage procedural risk
• plan post-treatment care and provide home-care recommendations
• identify the most common technical errors and consciously avoid them in practice

WHAT YOU RECEIVE IN THE LESSON

• Instructional video materials
• Interactive theoretical content
• Treatment protocols
• Product checklist
• Knowledge assessment quiz
• Access to a practice final exam
• Unlimited access to the lesson throughout the study period, with the option to extend access at any time