Polynucleotides & Exosomes

Polynucleotide Therapy Lesson

Polynucleotide therapy is an injectable procedure based on the administration of highly purified DNA/RNA fragments with regenerating, antioxidant, and deeply hydrating effects.

In this lesson, the student will learn about the chemical structure of nucleotides and the hydrophilic properties of polymers, as well as patient qualification, product selection (Soft, Medium, Strong), injection techniques, and the step-by-step sequence of action in accordance with the recommended treatment protocol.

What you’ll learn – theory

• Structure of polynucleotides
• Nucleotide structure
• Polynucleotide sourcing methods
• Safety and biocompatibility of preparations
• Classification by origin (DNA vs RNA)
• Classification by chain length and molecular weight
• Differences in chemical formulation (PDRN, PN-HPT™, CPN®)
• Therapeutic applications (Soft, Medium, Strong)
• Hydrophilic properties and water retention
• Extracellular matrix (ECM)
• Osmotic balance regulation
• Skin protection against oxidative stress
• Fibroblast and receptor activation
• Collagen, elastin, and hyaluronic acid
• Structure of cells and tissues
• Facial bone structure
• Facial vascular system
• Facial nerve system
• Facial muscles
• Skin anatomy
• Skin disorders
• Blood-borne diseases
• Effects of polynucleotide therapy
• Contraindications for the procedure
• Client qualification for the procedure
• Treatment area setup
• Products used in the procedure
• Step-by-step Nucleofill Strong application
• Post-treatment recommendations

What you’ll learn – practice

• Treatment area setup
• Materials needed for the procedure
• Client skin preparation
• Step-by-step Nucleofill Strong application

Curriculum update for 2025: This lesson will include a new topic – Exosomes. Expected release: October 2025. The update will be automatically available to all students with active access to the full-year program.