Polynucleotides & Exosomes

Polynucleotide Therapy Lesson

Polynucleotide therapy is based on the injection of highly purified DNA/RNA chains with regenerative, antioxidant, and hydrating properties. In this lesson, you’ll explore the chemical structure of nucleotides and polymers, their hydrophilic nature, and therapeutic uses. You'll also learn how to assess the client, choose the appropriate formula (Soft, Medium, Strong), perform the injection technique, and follow the treatment protocol step by step.

What you’ll learn – theory

• Understand the structure of polynucleotides – their physicochemical properties and role in tissue regeneration.
• Study the components of a nucleotide – nitrogen bases, sugars, and phosphate groups.
• Learn how polynucleotides are obtained – sources, purification process, and injectable formulation.
• Discuss the safety and biocompatibility of these preparations – including low immunogenicity and high tolerance.
• Learn the differences between DNA- and RNA-based formulations – and their regenerative effects.
• Understand the molecular weight and chain length variations between different products.
• Compare various formulations – PDRN, PN-HPT™, CPN® – and their clinical applications.
• Study how to use Soft, Medium, and Strong polynucleotide products – based on skin condition and client age.
• Explore their hydrophilic properties – and how they improve hydration by binding water.
• Understand the role of the extracellular matrix (ECM) – and how polynucleotides help rebuild it.
• Study osmotic balance mechanisms – and their impact on skin homeostasis.
• Learn how polynucleotides protect against oxidative stress – through antioxidant and stabilizing effects.
• Explore how fibroblasts and receptors are activated – stimulating collagen production and skin renewal.
• Understand the effects on collagen, elastin, and hyaluronic acid – the skin’s structural components.
• Review cell and tissue structure – to better understand the biological effects of treatment.
• Revisit facial bone anatomy – to support safe injection site planning.
• Study facial vascular structure – for safe product delivery.
• Understand facial innervation – to ensure maximum client comfort.
• Learn the facial muscle layout – for precision in anti-aging treatments.
• Explore skin anatomy – including layers and correct injection depths for polynucleotides.
• Review skin conditions – that may be contraindications for treatment.
• Revisit blood-borne diseases – and needle safety protocols.
• Learn the visible effects of polynucleotide therapy – improved elasticity, density, firmness, and tone.
• Study contraindications – including medical, dermatological, and healing-related risks.
• Understand client qualification – skin analysis, age, condition, and expectations.
• Prepare the treatment area – following aseptic and ergonomic guidelines.
• Review the necessary products – Nucleofill Strong, needles, gauze, disinfectants.
• Master the step-by-step Nucleofill Strong protocol – including method, depth, and injection points.
• Understand post-treatment care – skin hydration, reduced physical activity, and wellness recommendations.

What you’ll learn – practice

• Prepare your workstation – maintaining sterility, ergonomics, and safety.
• Get familiar with all materials – Nucleofill Strong, needles, gloves, gauze, protective items.
• Learn how to prepare the client’s skin – cleansing, disinfection, and injection point marking.
• Practice the full Nucleofill Strong procedure – following the protocol and aseptic technique.

Curriculum update for 2025: This lesson will include a new topic – Exosomes. Expected release: October 2025. The update will be automatically available to all students with active access to the full-year program.