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Lesson No 21
Carboxytherapy

Carboxytherapy Lesson
Carboxytherapy is an injection-based treatment involving intradermal or subcutaneous administration of medical-grade carbon dioxide (CO₂). It is used to improve microcirculation, oxygenation, and collagen production. In this lesson, you’ll learn the physiological mechanisms of CO₂ in the skin, proper injection techniques, safety protocols, and aesthetic standards.
What you’ll learn – theory
- Understand what carboxytherapy is – a procedure that involves injecting carbon dioxide for therapeutic and aesthetic purposes.
- Explore how CO₂ works in the tissues – boosting microcirculation, oxygenation, and stimulating collagen synthesis.
- Learn about the gas exchange process – how CO₂ affects local biochemical conditions and skin response.
- Recognize common skin reactions after treatment – including swelling, redness, mild pain, and a feeling of pressure.
- Review cell and tissue structure – to better understand biological responses to gas administration.
- Revisit facial bone anatomy – useful for identifying safe injection zones.
- Understand facial vascular anatomy – for effective and safe treatment application.
- Review facial nerve anatomy – to avoid sensitive areas and surface nerves.
- Learn about key facial muscles – relevant for skin-firming treatments.
- Deepen your knowledge of skin layers – focusing on target zones for CO₂ therapy.
- Identify skin disorders – both contraindications and indications for the procedure.
- Review blood-borne diseases and infection control principles.
- Learn safety and hygiene standards – including tool sterilization, disinfection, and waste disposal.
- Understand each step of the carboxytherapy procedure – from qualification to post-treatment care.
- Master gas injection techniques – including depth, angle, and application speed.
- Recognize contraindications – such as heart disease, cancer, pregnancy, or skin infections.
- Identify skin types and assess issues that can benefit from CO₂ therapy.
- Learn how to qualify clients – through consultation, indication review, and expectation assessment.
- Prepare your workspace – following aseptic techniques for carboxytherapy.
- Discover products used in the procedure – including needles, medical CO₂, and supporting formulas.
- Explore equipment and tools – types of devices, settings, and dosing systems.
- Reinforce safety measures – avoiding excessive pressure and monitoring skin reactions.
- Follow the carboxytherapy procedure step by step – from skin prep to post-treatment finish.
- Learn post-treatment guidelines – what to advise clients for optimal recovery and results.
- Combine carboxytherapy with other treatments – such as mesotherapy or facial massage.
What you’ll learn – practice
- Learn how to properly prepare your treatment area – with strict hygiene and safety standards.
- Get familiar with all required materials – needles, CO₂ device, gas, and protective supplies.
- Prepare the client’s skin – including cleansing, disinfection, and marking injection points.
- Practice the full carboxytherapy procedure – focusing on precision and client comfort.
Get your certificate after every course
After each course or lesson, you can test your knowledge with a quiz and instantly download a certificate in 3 languages. You’ll receive a diploma after completing the school and passing the final exam.








